In this video, Dr. Kendziorra delves into the science and methodology of various preservation strategies, expanding beyond traditional cryopreservation to a wider spectrum of methods designed for halting biological processes and ensuring cellular integrity over time. Explore the foundational methods of straight freezing and the advanced intricacies of vitrification, along with the innovative approaches of emergent vitrifixation and chemical fixation. Dr. Kendziorra explains the underlying principles, the specific challenges each method addresses, and the optimal conditions for their use. Learn about the role of cryoprotective agents in preventing ice crystal damage, the critical technique of perfusion for comprehensive tissue preservation, and the development of alternative strategies when conventional methods prove inadequate.